## Maximum number of birds

Total number of birds for any experiment was calculated using the Composite Number of Birds/Cage multiplied by 16 total isolation chambers.

## Composite Number of birds/cage

The composite number of birds allowed in one isolators was calculated for each week of age (0 to 52) using the lowest number of animals for any one of the default factors (floor space, linear feeders space, and drinker space) in the 2010 Ag Guide. Refer to the graphs below with the number of birds per cage based on individual factors (floor space, linear feeders space, and drinker space).

## Floor Space

The number of birds per cage based on floor space was calculated using average bodyweight and sizes for typical commercial breeds of broilers, layers, and turkeys, and the recommended sqin per bird in the 2010 Ag Guide. Each isolator has 855 sqin.

## Linear Feeder Space

The number of birds per cage based on linear feeder space was calculated using the recommendations in the 2010 Ag Guide. The feeder in the each isolator provides 22in of access to feed. If linear feeder space is the major impediment to bird numbers at a given age supplemental feed can be added provided it does not dramatically reduce useable floor space.

## Drinkers

The number of birds per cage based on access to water was calculated using the recommendations in the 2010 Ag Guide. The isolators can be configured to provide water through 2 nipples or 2 cups. The numbers of animals in each isolator varies considerably depending on which configuration is being used. Additionally, in the unlikely event that access to water is the limiting factor, supplemental water can be added using bell jars (or similar) provide it doesn't dramatically reduce the amount of usable floor space.